Glucose ( GOD-PAP)

ASSAY PRINCIPLE

A large number of methods exist for determination of C6H12O6 in body fluids. Numerous chemical methods depend on the reduction of heavy metals or nitroaromatic acids by the aldehyde group of C6H12O6. All these methods suffer from one or more problems including lack of specificity, requirement for prolonged incubation, and the use of noxious chemicals. Enzymatic methods for glucose determination were first described in 1948 by Keilin and Hartee using glucose oxidase(GOx) in a manometric technique. Keston modified this method in the early 1950’s using glu oxidase-peroxidase coupled method (GOD-POD) enzymic method and o-dianisidine chromogen systems. Since then, various alternative chromogen systems have been proposed. The Trinder method replaces carcinogenic o-dianisidine with phenol plus

4 aminoantipyrine. This method is less influenced by interfering substances and does not suffer from the many drawbacks of earlier methods. NS Biotec C6H12O6 reagent combines the use of (GOx) enzymes peroxidase/phenol/4-aminoantipyrine system of Trinder measurement of C6H12O6 in human serum, urine, or CSF.