INTENDED USE
NS Biotec ALT reagent is intended for the in vitro quantitative determination of alanine aminotransferase (EC 2.6.1.2) activity in serum on both automated and manual systems.
ASSAY PRINCIPLE
Alanine aminotransferase methods using U.V. procedures were first described by Henley in 1955. Wroblewski and LaDue in 1956 described a method for determining alanine aminotransferase using lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide, reduced (NADH). This method was later modified by Henry and Bergmeyer to optimize substrate conditions and eliminate side reactions. The NS Biotec alanine aminotransferase reagent is based on the recommendations of the IFCC.
Other features:
• Kinetic method
• Liquid ready-to-use reagents
• Stable to expiry when stored at 2-8◦C



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