INTENDED USE
NS Biotec ALT reagent is intended for the in vitro quantitative determination of alanine aminotransferase (EC 2.6.1.2) activity in serum on manual systems.
ASSAY PRINCIPLE
Colorimetric methods based on formation of the chromogenic dinitrophenylhydrazone of pyruvate have been in wide use. However, the accuracy of these methods is limited, and since dinitrophenylhydrazine reacts with -ketoglutarate as well as pyruvate, high reagent blanks are obtained.
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