Brucella-A&M (Qualitative &Semi Quantitative)

PRINCIPLE:
The smooth, attenuated stained Brucella antigen suspensions are mixed with the patient’s serum. Specific antibodies to Brucella antigens if present in the patient serum will react with the antigen suspension to produce an agglutination reaction. No agglutination indicates the absence of specific antibodies to Brucella antigens Human Brucellosis (Diumal, or undulant fever) is a common febrile illness caused by infection with bacteria of some of the Brucella species (abortus, melitensis). This undulant fever, sweats and anorexia. On exposure the body responds to this antigenic stimulation by producing specific antibodies whose titres rise slowly at early stages and then increases. Specific antibodies to the Brucella spcies are detectable a few weeks after exposure and are of considerable importance in the diagnosis of Brucellosis.