INTENDED USE
NS Biotec calcium reagent is intended for the in vitro quantitative determination of calcium in human serum, plasma and urine on both automated and manual systems.
ASSAY PRINCIPLE
Many colorimetric methods have been developed for the determination of calcium. These methods include colorimetric, fluorescent, gravimetric, ion selective, titrimetric, and atomic absorption techniques.
Connerty and Briggs described methods using alizarin 3-sulphonate and cresolphthalein complexone5 whilst Gindler and King have described a method using thymol blue. There have been many subsequent modifications to these methods. NS Biotec calcium reagent is based on the cresolphthalein complexone (CPC) method of Moorehead and Briggs1. CPC reacts with calcium and magnesium in alkaline solution to form a deeply coloured complex. 8-hydroxyquinoline is incorporated into the reagent to preferentially bind magnesium and prevent interference from this cation. CPC is an acid-base indicator necessitating the use of a strong buffer to stabilize the pH.
Ca reacts with cresolphthalein complexone to form purple color complex in alkaline medium.
The intensity of color measured photometrically between 540 and 600 nm with maximum absorbance at 575 nm is directly proportional to ca concentration in the specimen.
Reagent Stability
All reagents are stable up to the expiry date given on label when stored at room temperature.
Reviews
There are no reviews yet.