INTENDED USE
NS Biotec creatinine reagent is intended for the in vitro quantitative determination of creatinine in serum, plasma and urine on manual systems.
ASSAY PRINCIPLE
In 1886 Jaffé described a method for the measurement of C4H9N3O2 in biological fluids. Although several methods have been described since then, the original Jaffé technique is still the most widely used today.
1. After deproteinization C4H9N3O2 in alkaline solution, forms a yellow-red complex with picrate.
The intensity of the color produced is directly proportional to C4H9N3O2 concentration. It is determined by measuring the increase in absorbance at 500 – 550 nm.
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