INTENDED USE
NS Biotec Alpha Amylase reagent is intended for the in-vitro quantitative, diagnostic determination of Alpha-amyl_C2 in human serum , heparinized plasma or urine on both automated and manual systems.
Assay Principle
Alpha-amyl_C2 catalyzes the hydrolysis of 2-chloro-4-nitrophenyl- 1- galactopyranosyl-maltoside (Kinetic) to glucose polymers and p- nitrophenyl oligosaccaride at short chain producing 2-chloro- 4- nitrophenol (CNP).
The increased extinction can be measured by spectrophotometry at 405 nm and results are proportional to the activity of alpha amylasepresent in the sample.
Method
Kinetic or Fixed Rate method – Kinetic
Background
Amylase is found primarily in the pancreas and salivary glands. When released in the digestive tract, the enzyme hydrolyzes starch. Amylase determinations are useful in the diagnosis and treatment of diseases of the pancreas and parotids. Elevated serum levels areassociated with acute pancreatitis and other pancreatic disorders as well as mumps and bacterial parotitis.
Reagent Preparation Storage and Stability
Amylase reagents are supplied ready-to-use and stable till the expiration date labeled on the bottles when properly stored refrigerate 2- 8°C. Once opened, the reagent is stable for 2 months at the specified temperature.
Precautions
Do not ingest or inhalate. In case of contact with eyes or skin; rinse immediately with plenty of soap and water. In case of severe injuries;seek medical advice immediately.
Saliva and skin contain alpha amylase: never pipette by mouth and avoid skin contact with the reagents (use gloves).Avoid use of hemolysed samples.
The present method describes the manual use of this kit . For use with automatic analyzer see the specific applications.
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