Creatine Kinase ( CK )

INTENDED USE

NS Biotec creatine kinase reagent is intended for the in vitro quantitative determination of Creatine Kinase (CK) in human serum and plasma.

ASSAY PRINCIPLE

The assay method using creatine phosphate and ADP was first described by Oliver, modified by Rosalki and further improved for optimal test conditions by Szasz. CK is rapidly inactivated by oxidation of the sulfhydryl groups in the active center. The enzyme can be reactivated by the addition of acetylcysteine (NAc). Interference by adenylate kinase is prevented by the addition of diadenosine pentaphosphate and AMP. Standardized methods for the determination for CK using the “reverse reaction” and activation by NAc were recommended by the German Society for Clinical Chemistry (DGKC) and the International Federation of Clinical Chemistry (IFCC) in 1977 and 1990 respectively. This assay meets the recommendations of the IFCC and DGKC (Standard method 94).