INTENDED USE
NS Biotec inorganic phosphorus reagent is intended for the in vitro quantitative determination of inorganic phosphorus in human serum, plasma and urine on both automated and manual systems
CLINICAL SIGNIFICANCE
The body of human adult contains approximately 620 g of phosphorus entirely in the form of phosphates distributed fairly equally between extracellular and intracellular compartments. About 85 % of extracellular phosphate occurs in inorganic forms as hydroxyapatite. In plasma or serum, most phosphate exist in the inorganic form (Pi); this fraction is present as the mono-and dihydrogen forms, the relative proportions varying with the pH. Intracellularly, phosphate occurs mainly as phospholipids and phosphoproteins; this fraction is termed organic phosphate. Increased serum phosphate levels may occur in renal failure, hypervitaminosis D,and hypoparathyroidism. Reduced serum phosphate levels are seen in vtamin D deficiency, rickets, hyperparathyroidism, and fanconi’s syndrome.
ASSAY PRINCIPLE
Inorganic phosphate reacts with ammonium molybdate in presence of sulfuric acid to form nonreduced phosphomolybdate.
Reagent Preparation & Stability
All reagents are ready for use and stable up to the expiry date given on label when stored at 2-8°C.
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