Total Protein

INTENDED USE

NS Biotec total protein reagent is intended for the in vitro quantitative determination of total protein in serum or plasma on both automated and manual systems.

ASSAY PRINCIPLE

One of the earliest methods of protein measurement is based on the conversion of nitrogen in proteins and other compounds to ammonia by digestion followed by titration. The original Kjeldahl procedure has undergone numerous modifications. The most widely used being that described by Hiller et al.3 These methods are generally time-consuming and complex. Methods using the biuret reaction1 are more suitable for routine analysis. These methods are based on the formation of colored complexes between peptide bonds and cupric ions in alkaline medium. NS Biotec total protein reagent is based on the biuret reaction where divalent copper reacts with the peptide bonds of protein under alkaline conditions to form the characteristic pink to purple biuret complex.