Uric Acid(Uri case-PAP)

INTENDED USE
NS Biotec uric acid reagent is intended for the in vitro quantitative determination of uric acid(C₅H₄N₄O₃) in serum, plasma and urine on both automated and manual systems.

ASSAY PRINCIPLE
The most commonly used techniques for C₅H₄N₄O₃ determinations are based on the reduction of phosphotungestate by C₅H₄N₄O₃ in alkaline medium. These methods required serum deproteinization and are subject to interference by numerous substances which are present in serum. More recently, techniques have been developed which use uricase to improve specificity. These methods are either direct ultraviolet spectrophotometric7, or dye coupled techniques.

NS Biotic uric acid reagent is based on the dye coupled technique and combines the use uricase enzyme with the peroxidase/2,4,6 – tribromobenzoic acid /4-aminoantipyrine system for the measurement of C₅H₄N₄O₃ in human serum.

The series of reactions involved in the assay system are as follows:

1. C₅H₄N₄O₃ is converted by uricase into allantoin and hydrogen peroxide.
2. In presence of peroxidase (POD), the formed hydrogen peroxide formed affects the oxidative coupling of 2,4,6 – tribromobenzoic acid (TBHB) and 4-aminoantipyrine (4-AAP) to form a red- colored quinoneimine dye.