INTENDED USE
NS BIO-TEC diagnostics Lipase-LS reagent is intended for in-vitro quantitative determination of Lipase in human
serum, heparinized or EDTA plasma.
PRINCIPLE
A synthetic substrate (DGMRE) is split by Lipase to yield the Colored final product Methylresorufin. The increasing Absorbance of the red Methylresorufin is measured photometrically .
The reaction is highly specific on the human enzyme.
Background
Pancreatic lipase in serum is closely associated with Pancreatic diseases. The activity of this enzyme has been Measured as an important marker for diagnosing pancreatic Diseases and the associated monitoring of therapeutic effects.Pancreatic lipase test kits currently available include a turbidimetric method using triglyceride as substrate and a Colorimetric method using synthetic substrates.
Expected values
< 60 U/l
Note: It is recommended for each laboratory to establish and maintain its own reference values. The given data are only an indication.
Calibrators and control
For the calibration of automated analyzers Q-SLAP Multicalibrator is recommended, for quality control use NS BIO-TECnormal and abnormal controls.
Sensitivity
The detection limit is equal to 3 U/l.
Stability
When stored at 2-8° C and protected from light,
The reagents are stable up to the expiry date printed on the labels.
Preparation and stability of Working reagents
The reagents are ready to use.
Stability: 3 months at 2-8°C, if Contamination is avoided
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